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Welcome to the HiSeq2000 HT Sequencing Page

The IIGB Genomics Core facility provides a next-generation sequencing service using Illumina's newest HiSeq2000 platform. Combined with the latest in cluster generation and sequencing reagents, the HiSeq2000 delivers much greater yields than the GAIIX. It achieves this due to improved flowcell and hardware design and the ability to run two flowcells independently and simultaneously doubling our throughput and reducing wait times. The HiSeq2000 can generate genome, small RNA and transcriptome level sequences with unprecedented speed for a cost per cycle that is less than that of the older GAIIX. The most common research applications of this technology are:

Genome-wide detection of SNPs and mutations DNA methylation profiling DNA-protein interactions RNA expression profiling Small RNA profiling and discovery ChIP sequencing

Service Overview

A deep sequencing project consists of three major steps: the library synthesis, the sequencing run and the data analysis. Currently, the facility expects users to construct libraries for the sequencing runs. There are numerous commercial kits available for libraries and multiplex sequencing. The Deep Sequencing team can help you choose the most appropriate preparation method for your project. The Deep Sequencing Protocol page provides detailed information about the library types and their synthesis with kits from Illumina. The actual sequencing with the HiSeq2000 is performed by our staff in the IIGB Genomics Core facility. To submit libraries, please follow the instruction on the Sample Submission page. The available bioinformatics services for the downstream data analysis are described on the Data Analysis page.

Contacts

Genomics (libraries, HiSeq2000)

• Glenn Hicks, IIGB Academic Administrator
• John Weger, Specialist, Genomics
  
• Barbara Walter, Staff Research Associate

Bioinformatics

• Thomas Girke, Director, IIGB Bioinformatic Facility
• Rebecca Sun, Bioinformatics Analyst