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Welcome to the HT Sequencing Page

The IIGB Genomics Facility provides a next-generation sequencing service using the Illumina Genome Analyzer II platform. This deep sequencing technology can generate genome and transcriptome level sequences with unprecedented speed for very low cost. The most common research applications of this technology are:

Genome-wide detection of SNPs and mutations DNA methylation profiling DNA-protein interactions RNA expression profiling Small RNA profiling and discovery

Service Overview

A deep sequencing project consists of three major steps: the library synthesis, the sequencing run and the data analysis. Currently, the facility expects users to synthesize the libraries for the sequencing runs. The Deep Sequencing Protocol page provides detailed information about the library types and their synthesis with kits from Illumina. The actual sequencing with the Genome Analyzer II is performed by our staff in the IIGB Genomics facility. To submit libraries, please follow the instruction on the Sample Submission page. The available bioinformatics services for the downstream data analysis are described on the Data Analysis page.

Contacts

Molecular Biology

• Glenn Hicks, IIGB Academic Administrator
• Barbara Walter, Staff Research Associate

Bioinformatics

• Thomas Girke, Director, IIGB Bioinformatic Facility
• Tyler Backman, Bioinformatics Analyst
  
• Rebecca Sun, Bioinformatics Analyst